Such multiple genes or epitopes can be included in a single vector, for example a plasmid, or in multiple plasmids, each of which must be transformed into the algae. The gene fusion then was cloned into the multi-cloning site of plasmid pSSCR7 under control of the β2 tubulin promoter of Chlamydomonas. Three independent clones stably transformed with pBR26 were mated, pairwise to 3 independent clones expressing β-glucuronidase (GUS). Aquatic invertebrates which are suitable host animals include, but are not limited to, shrimp, crabs, oysters, and clams. A potential disadvantage of the double 2A vector is that the 2A C-terminal fusion to the middle protein of the poly-cistron may disrupt its function and/or localization. In algae auxotrophic strains and gene complementation for the nitrate reductase gene (Kindle) and a uracil biosynthetic gene (URA3) (Takemura) are available. A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae. The following examples are for purposes of illustration only and are not intended to limit the scope of the invention as defined in the claims which are appended hereto. (A) Relevant restriction sites delineating the rbcL 5′ UTR (BamHI/NdeI), the HSV8 coding region and flag tag (NdeI/XbaI), and the rbcL 3′ UTR (XbaI/BamHI), as well as relevant restriction sites of the atpA 5′ UTR (BamHI/NdeI), the HSV8 coding region and flag tag (NdeI/XbaI), and the rbcL 3′ UTR (XbaI/BamHI) are shown. Within the context of this application, such algae are said to be “disabled.” Use of such disabled strains inhibits or limits spread of the transgenic algae of the present invention into the environment. Triacylglycerol (TAG) is also a storage compound but it is stored in lipid droplets, while the mechanisms for controlling flux and accumulation of each compound is still under study. Most of the progeny from both crosses displayed significantly more GUS activity than the parent strains. 14. In contrast, no 57 kD proteins were detected in serum from fish that were immersed in either E-22, CP57, wild-type or no algae. All the fish groups were fed with the same commercial diet at the rate of 2% of fish weight for 3 weeks (Bioproducts, Inc. Oregon). Weight, Spleen Relative Weight (SRW), Hematocrit After the 4 days of the 2nd oral treatment, all the fish groups were fed the same commercial diet for 4 weeks until the second sampling in this experiment. These results demonstrate the utility of alga as an expression platform for recombinant proteins, and describe a new type of single chain antibody containing the entire heavy chain protein, including the Fc domain. and Hemoglobin of Fish Treated Orally Cell lines expressing both ER-mCherry and nuclear mCerulean were crossed with cells transformed with mitochondria-targeted Venus (D), to obtain progeny that stably expressed three distinct FPs in three sub-cellular locations (E). In addition mature T cells display one of two accessory molecules, CD4 or CD8. Wong, M.H. https://doi.org/10.1093/pcp/pcy156. The concentrations of the three types of algae were as follows: CP57, 7.97×105 cells/ml; 2137, 2.71×106 cells/ml; and E-22, 2.97′ 106 cells/ml. E. Live cell microscopy of a cell line transformed with pBR32, chloroplast-targeted mCherry (red). SRW1 (1994) Plant Cell 6:53-63). Base-pairs of nucleotides not used in nature are being incorporated into synthetic organisms, which are equally capable storing and presumably expressing genetic information (Hirao). As used herein the term “host animal” refers to all animals capable of mounting an immune or defense response when infected with a pathogenic microorganism. This system has many similarities to the nonspecific defense system of vertebrates, such as the activation of phagocytotic cells (hematocytes). Plasmids are introduced into the algae by standard transformation methods known to those skilled in the art, such as for example, electroporation, vortexing cells in the presence of exogenous DNA, acid washed beads, polyethylene glycol, and biolistics. There are an expanding number of gene editing techniques becoming available for microalgae and the efficacy and advantages of each are not always clear. The repertoire is generated by combinatorial joining of variable (V), joining (J), and diversity (D) genes, and by N region (nucleotides inserted by the enzyme deoxynucleotidyl-transferase) diversification.